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  • 净化车间降噪处理要注意什么?

    来源:http://www.jnyhrs.com/ 发布时间:2021-12-22
    试剂储存和准备区的功能是:贮存试剂的制备、试剂的分装和扩增反应混合液的准备,以及离心管、吸头等消耗品的贮存和准备。贮存试剂和用于标本制备的消耗品等材料应当直接运送至试剂贮存和准备区,不能经过扩增检测区,试剂盒中的阳性对照品及质控品不应当保存在该区,应当保存在标本处理区。
    The function of reagent storage and preparation area is to store the preparation of reagents, the dispensing of reagents and the preparation of amplification reaction mixture, as well as the storage and preparation of consumables such as centrifuge tubes and suction heads. Materials such as stored reagents and consumables used for sample preparation shall be directly transported to the reagent storage and preparation area, and shall not pass through the amplification detection area. The positive control and quality control products in the kit shall not be stored in this area, but shall be stored in the sample processing area.
    标本制备区的功能是:核酸(RNA、DNA)提取、贮存及其加入至扩增反应管。对于涉及临床样本的操作,应符合生物安全二级实验室防护设备、个人防护和操作规范的要求。
    The function of the sample preparation area is to extract and store nucleic acids (RNA and DNA) and add them to the amplification reaction tube. Operations involving clinical samples shall meet the requirements of biosafety level II laboratory protective equipment, personal protection and operation specifications.
    由于在样本混合、核酸纯化过程中可能会发生气溶胶所致的污染,可通过在本区内设立正压条件,避免从邻近区进入本区的气溶胶污染。为避免样本间的交叉污染,加入待测核酸后,必须盖好含反应混合液的反应管。对具有潜在传染危险性的材料,必须在生物安全柜内开盖,并有明确的样本处理和灭活程序。
    Since aerosol pollution may occur during sample mixing and nucleic acid purification, positive pressure conditions can be set in this area to avoid aerosol pollution from adjacent areas. To avoid cross contamination between samples, the reaction tube containing the reaction mixture must be covered after adding the nucleic acid to be tested. For materials with potential infectious risk, the cover must be opened in the biosafety cabinet, and there are clear sample handling and inactivation procedures.
    PCR实验室净化装修
    扩增区的功能是:DNA合成、DNA扩增及检测。为避免气溶胶所致的污染,应当尽量减少在本区内的走动。必须注意的是,所有经过检测的反应管不得在此区域打开。
    The function of amplification region is: DNA synthesis, DNA amplification and detection. In order to avoid pollution caused by aerosols, it is necessary to minimize walking in the area. It must be noted that all tested reaction tubes must not be opened in this area.
    扩增产物分析区的功能是:扩增片段的进一步分析测定,如杂交、酶切电泳、变性液相分析、测序等。核酸扩增后产物的分析方法多种多样,如膜上或微孔板或芯片上探针杂交方法(放射性核素标记或非放射性核素标记)、直接或酶切后琼脂糖凝胶电泳、聚丙烯酰胺凝胶电泳、Southern转移、核酸测序方法、质谱分析等。
    The function of the amplified product analysis area is to further analyze and determine the amplified fragments, such as hybridization, enzyme digestion electrophoresis, denaturing high-performance liquid analysis, sequencing, etc. There are various analysis methods for the products after nucleic acid amplification, such as probe hybridization method (radionuclide labeling or non radionuclide labeling) on membrane or microplate or chip, agarose gel electrophoresis, polyacrylamide gel electrophoresis, southern transfer, nucleic acid sequencing method, mass spectrometry analysis, etc.
    扩增产物分析区是PCR实验室主要的扩增产物污染来源,因此应注意避免通过本区的物品及工作服将扩增产物带出。在使用PCR-ELISA方法检测扩增产物时,必须使用洗板机洗板,废液必须收集至1mol/LHCl中,并且不能在实验室内倾倒,而应当至远离PCR实验室的地方弃掉。用过的吸头也必须放至1mol/LHCl中浸泡后再放到垃圾袋中按程序处理,如焚烧。由于本区有可能会用到某些可致基因突变和有毒物质如溴化乙锭、丙烯酰胺、甲醛或放射性核素等,故应当注意实验人员的安全防护。
    The amplification product analysis area is the main source of amplification product pollution in the PCR laboratory. Therefore, attention should be paid to avoid taking the amplification products out through the articles and work clothes in this area. When the PCR-ELISA method is used to detect the amplification products, the plate washing machine must be used to wash the plate. The waste liquid must be collected into 1mol / lhcl and cannot be dumped in the laboratory, but should be discarded away from the PCR laboratory. The used suction head must also be soaked in 1mol / lhcl and then put into the garbage bag for treatment according to the procedure, such as incineration. Since some mutagenic and toxic substances such as ethidium bromide, acrylamide, formaldehyde or radionuclides may be used in this area, attention should be paid to the safety protection of laboratory personnel.
    此外要知道PCR实验室净化装修的核心关键技术是气流控制、避免交叉污染,而不是空气洁净,空气洁净增加了工程造价和运行费用,对气流控制、避免交叉污染没有任何帮助。更多相关内容就来我们网站http://www.jnyhrs.com了解吧。
    In addition, we should know that the core key technology of PCR gene amplification laboratory is air flow control and avoid cross pollution, not air cleaning. Air cleaning increases project cost and operation cost, and does not help air flow control and avoid cross pollution. For more information, please visit our website http://www.jnyhrs.com Understand.
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