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  • 无菌室无菌环境的净化控制!
    来源:http://www.jnyhrs.com 浏览: 发布日期:2019-08-16
      Quality control of aseptic environment in aseptic room is very important for obtaining accurate microbial data.
      Food microbiological testing has strict requirements for aseptic environment and aseptic operation. The whole detection process always requires aseptic operation in aseptic state, so the quality control of aseptic state is the guarantee to ensure the accuracy and reliability of microbial detection results.
      Operators should regularly and strictly evaluate the disinfection effect of sterile rooms. At the same time, reasonable environmental monitoring procedures should be established. Data analysis of environmental monitoring results should be acceptable. The number of bacterial colonies in different working areas should be strictly in accordance with the corresponding national standards, and records should be recorded to control the treatment exceeding the standard.
      In addition, the sterile room environment, such as temperature and humidity, may affect the disinfection and sterilization effect. Generally, the temperature is controlled at about 22 C, and the relative humidity is not more than 65%.
      The whole sterile room should adopt advanced and efficient laminar air purification system to ensure the requirements of constant temperature, humidity, ventilation and air cleanliness. The high efficiency filter membrane in the air purification system can effectively filter dust particles of different sizes and specifications in the air, and prevent microorganisms adhering to dust particles from polluting the sterile chamber. Air distribution from top to bottom ensures cleanliness and energy conservation.
      Ultraviolet bactericidal lamp is installed in sterile room, personnel passageway and logistics window, which can disinfect the surface of articles and air. When working, the highly efficient filtered air in the air shower room can blow off some dust attached to the operator's work clothes, and the logistics window can transfer laboratory supplies and samples. All these designs ensure the cleanliness of the sterile room.
      At the same time, the air volume of HEPA filter system can meet the needs of personnel, better solve the problems of traditional sterile room can not replace fresh and clean air, poor working environment and so on.
      In addition, the aseptic degree of the sterile room should be monitored regularly. According to the limits of dust particles, sedimentation bacteria and plankton bacteria in the clean room of pharmaceutical industry, the food microbial aseptic room can be detected regularly.
      解决的方法是最简单和直观的,能真正反映最表面(特别是控制区)的自然和污染特征,因此,也可以用平皿计数琼脂和孟加拉红琼脂板3块,等距离放置在一个无菌室工作位置在对角线上,打开暴露15分钟,分别在36℃培养48 h, 28℃培养5 d数结算后的细菌和霉菌和酵母数量的殖民地,如果菌落平均计数超过3 cfu/盘,应对无菌室内空气和表面进行消毒,以改善其无菌环境。
      The solution is the simplest and intuitive, which can truly reflect the nature and pollution characteristics of the most surface (especially the control area). Therefore, agar plate and Bangladesh red agar plate can also be counted with a dish. They can be placed at a diagonal working position in an aseptic chamber at equal distances, exposed for 15 minutes, incubated at 36 for 48 hours and incubated at 28 for 5 days. If the average count of bacteria, fungi and yeast exceeds 3 cfu/disc, the sterile indoor air and surface should be disinfected to improve the sterile environment.
      Effective biosafety systems and strict enforcement should be established in the microbial sterility room, such as putting pathogenic bacteria into the biosafety cabinet to install transparent and visible glass doors, pasting corresponding safety level "biological hazard" icons on the doors, and clearing contact information with emergency contacts.
      Special garbage cans that can be automatically closed should be placed in different areas, and warning labels such as "indoor ultraviolet disinfection lamp" should be affixed to areas with ultraviolet radiation such as logistics windows.