Aseptic room, also known as inoculation room, is a special laboratory for aseptic operations such as systematic inoculation and purification of bacteria. In microbial work, inoculation and transplantation of strains is a main operation. The characteristic of this operation is to ensure pure strains and prevent contamination by miscellaneous bacteria. In the air of the general environment, due to the existence of many dust and miscellaneous bacteria, it is easy to cause pollution and greatly interfere with the vaccination work. Iwuchen introduces the standardization of decoration design of microbial sterile room.
Aseptic rooms should be set up according to both economic and scientific principles. The basic requirements are as follows:
(1) The aseptic room should have two rooms, one inside is aseptic room and the other outside is buffer room. The room volume should not be too large to facilitate air sterilization. Internal area 2 × 2.5 ＝ 5m2, external area 1 × 2 ＝ 2M2, the height should be less than 2.5m, and there should be a ceiling.
(2) Sliding door shall be set in the interior to reduce air fluctuation, and the door shall be set at the farthest position from the workbench; It is better to use sliding door for the door of the outer room, which shall be set at the farthest position from the inner room.
(3) A small window shall be opened on the wall or "partition" separating the inner and outer rooms as a necessary channel for internal and external transfer of goods during the vaccination process, so as to reduce the number of personnel entering and leaving the inner room and reduce the degree of pollution. The small window is 60cm wide, 40cm high and 30cm thick, and opposite sashes are hung inside and outside.
(4) The volume of the sterile room is small and tight. After use for a period of time, the indoor temperature is very high, so a ventilation window should be set. The ventilation window shall be set on the ceiling at the entrance of the inner room (i.e. the farthest position from the workbench), preferably double-layer structure, the outer layer is louver, and the inner layer can use extraction plate sash. The ventilation window can be opened after the inner chamber is used and before sterilization to circulate air. If possible, a constant temperature and humidity machine can be installed.