Key points of plane planning of sterile room
Taking the aseptic Laboratory of a pilot test base of medical testing products contracted by ceidi Xidi as an example. In order to meet the sterility requirements, when ceidi Xidi planned the sterile room, the entrance avoided the corridor and was set in the bacterial test operation room. The sterile room and the operation room are separated by two buffers, and a double-layer window is set between the two rooms to form a small channel.
The entrance of sterile laboratory shall be equipped with purification facilities for personnel and materials, such as hand washing facilities and dressing room.
Key points of HVAC and lighting in sterile room
The temperature of aseptic laboratory should be controlled between 15 ~ 27 ℃ and the humidity should be controlled below 50%.
The sterile laboratory shall be equipped with effective ventilation equipment to make the air flow from the clean area to the non clean area. When mechanical ventilation is adopted, the air exchange rate shall be greater than 3 times / hour.
In the sterile room, ceidi Xidi has installed a 30W UV lamp every 3 square meters to achieve sterilization. In addition to ultraviolet lamps, asepsis room can also use fumigation and spray these two ways to sterilize. When the aseptic room is used for a long time or seriously polluted, fumigation can be used, which is a measure of thorough sterilization; Spraying and ultraviolet sterilization are carried out every time before using the asepsis room. Normally, the ultraviolet lamp can irradiate 30~60min to achieve a good bactericidal effect.
Analysis on key points of decoration of microbial aseptic laboratory in Shanghai Bay Area biomedical port
Only by maintaining a relatively sterile environment can the aseptic room study a specific known microorganism, provide protection for samples, ensure the accuracy of experimental results and even the life safety of experimental personnel. Therefore, the ability and stability of bacteria control are the core acceptance indicators of the sterile room. The accepted standard in the industry is that the average number of miscellaneous bacteria on the plate in class 100 clean area shall not exceed 1 colony, and the average number of bacteria in class 10000 clean room shall not exceed 3 colonies. If it exceeds the limit, the microbial sterile laboratory shall be thoroughly disinfected until the repeated inspection meets the requirements.