The aseptic room purification project is a new technology used to ensure that the experimental process in the laboratory is not contaminated by external pollution, so that more accurate and reliable research results can be obtained. This article will introduce the basic concepts, engineering processes, and key points of aseptic room purification engineering.

一、无菌室的定义

1、 Definition of sterile room

无菌室属于实验室生物安全层级三的实验室，它是一种集过滤、通风、消毒于一体的洁净室。主要包括两种类型：垂直流无菌室和水平流无菌室。它可以用于生物制品、微生物学、细胞学、分子生物学、生化学等实验。

The sterile room belongs to the third level of laboratory biosafety, and is a clean room that integrates filtration, ventilation, and disinfection. It mainly includes two types: vertical flow sterile room and horizontal flow sterile room. It can be used in biological products, microbiology, cytology, molecular biology, biochemistry, and other experiments.

二、无菌室净化工程的流程

2、 Process of aseptic room purification engineering

无菌室净化工程的流程主要包括设计、建造、验证和维护四个过程。其中，设计和建造是进行无菌室净化工程的前提，验证和维护是无菌室正常运行的必须环节。

The process of aseptic room purification engineering mainly includes four processes: design, construction, validation, and maintenance. Among them, design and construction are the prerequisites for carrying out the sterile room purification project, and verification and maintenance are necessary steps for the normal operation of the sterile room.

1. 设计阶段

1. Design stage

无菌室的设计要根据实验环境和操作需求来设计操作类型、封闭性、空气流量和空气处理系统等，从而保证无菌室能够符合实验要求。

The design of a sterile room should be based on the experimental environment and operational requirements to design the type of operation, closure, air flow, and air handling system, so as to ensure that the sterile room can meet experimental requirements.

2. 建造阶段

2. Construction stage

建造无菌室的过程包括对墙体、地面、天花板和门窗的设计和建造，以及空气处理和净化系统的建造和安装。

The process of building a sterile room includes the design and construction of walls, floors, ceilings, and doors and windows, as well as the construction and installation of air handling and purification systems.

3. 验证阶段

3. Validation stage

验证是确保无菌室能够符合设计要求的重要步骤，其中包括以下方面：

Validation is an important step to ensure that a sterile room can meet design requirements, including the following aspects:

(1)确认气体流量

(1) Confirm gas flow

(2)确认无菌室内的背景污染

(2) Confirm the background pollution in the sterile room

(3)评估过滤器的效率

(3) Evaluate the efficiency of the filter

(4)评估洁净度等

(4) Evaluate cleanliness, etc

4. 维护阶段

4. Maintenance phase

无菌室的维护包括日常维护和定期维护两部分。日常维护主要包括：定期更换过滤器、进行无菌室的清洁和消毒，定期测量空气质量等。定期维护主要包括：对空气处理和净化系统的检修和维护，对过滤器和空气处理系统进行更换或升级等。

The maintenance of the sterile room includes routine maintenance and regular maintenance. Routine maintenance mainly includes regular replacement of filters, cleaning and disinfection of sterile rooms, and regular measurement of air quality. Regular maintenance mainly includes: overhaul and maintenance of air handling and purification systems, replacement or upgrading of filters and air handling systems, etc.

三、无菌室净化工程的要点

3、 Key points of aseptic room purification engineering

1. 保证无菌室的净化水平。建造无菌室时，要保证墙体、地面、门窗、空气处理系统等的质量，以满足对无菌室的净化要求。另外，每天要进行日常清洁消毒操作来保证无菌室的洁净度。

1. Ensure the purification level of the sterile room. When constructing a sterile room, it is necessary to ensure the quality of walls, floors, doors and windows, and air handling systems to meet the purification requirements for the sterile room. In addition, daily cleaning and disinfection operations should be carried out every day to ensure the cleanliness of the sterile room.

2. 保证空气平衡。控制空气流量和空气质量可保持粒子和微生物的污染降至，避免污染物在室内空气循环中积累。

2. Ensure air balance. Controlling air flow and air quality can minimize particle and microbial pollution and avoid the accumulation of pollutants in the indoor air circulation.

3. 定期更换过滤器。无菌室的空气处理系统中过滤器的质量对无菌室的净化效率有很大影响，每隔一定时间要对过滤器进行更换和清洁。

Replace the filter regularly. The quality of filters in the air handling system of a sterile room has a significant impact on the purification efficiency of the sterile room. Filters should be replaced and cleaned at regular intervals.

4. 建立严格的操作规范。无菌室的操作人员必须持证上岗，接受专门的操作规范培训，按照标准操作流程进行实验操作。

4. Establish strict operating specifications. Operators of the sterile room must hold a certificate, receive specialized training in operating specifications, and conduct experimental operations in accordance with standard operating procedures.

总之，无菌室净化工程是一项很重要的工程，其目的是为了保证实验的准确性和安全性。当然，在建造和操作无菌室时还需要考虑更多的因素，如环境压力、安全控制、废物管理等。相信随着科技的不断发展，无菌室的净化技术也会不断创新和完善。

In short, the aseptic room purification project is a very important project, whose purpose is to ensure the accuracy and safety of the experiment. Of course, more factors need to be considered when constructing and operating sterile rooms, such as environmental pressure, safety control, waste management, and so on. I believe that with the continuous development of science and technology, the purification technology of sterile rooms will continue to innovate and improve.