Sterile laboratories should use 0.1% benzalkonium bromide solution or 2% formaldehyde solution before and after each use to wipe the workbench and potential contaminated dead corners, wet mop the ground, turn on the sterile air filter and the switch of the ultra clean workbench for 30 minutes, and irradiate the ultraviolet sterilization lamp for 1 hour.

操作人员用肥皂洗手后进入缓冲间换拖鞋，用百分之75酒精棉球擦手，穿戴工作衣帽口罩。将所需物品剥去牛皮纸及外包装，从传递窗移入操作间，再用百分之0.1苯扎溴铵溶液泡手后进入操作间，在酒精(或煤气灯)火焰区(火焰高度3-5cm)操作。

After washing hands with soap, the operator enters the buffer room to change slippers, wipes their hands with a 75% alcohol cotton ball, and wears work clothes, hats, and masks. Peel off the kraft paper and outer packaging of the required items, transfer them from the transfer window to the operation room, and then soak them with 0.1% benzalkonium bromide solution before entering the operation room. Operate in the alcohol (or gas lamp) flame area (flame height 3-5 cm).

操作者不得用嘴直接吸吹吸管，防止致病菌感染操作人员及口中的杂菌污染供试品。

The operator is not allowed to directly blow the straw with their mouth to prevent pathogenic bacteria from infecting the operator and contaminating the test sample with impurities in their mouth.

供试品表面消毒，如为安瓿先用砂轮划痕后用百分之2碘酒棉球擦拭，再用百分之75酒精棉球擦拭待干，也可以用消毒液浸泡消毒。

Disinfect the surface of the test sample. If the ampoule is scratched with a grinding wheel, wipe it with a 2% iodine alcohol cotton ball, and then wipe it with a 75% alcohol cotton ball to dry. Alternatively, soak it in disinfectant for disinfection.

注射器如需回抽时，应对着火焰吸入无菌空气。

When the syringe needs to be retracted, sterile air should be inhaled into the flame.

在无菌操作中切勿大幅度或快速动作拖行，以免搅动空气中尘埃微粒。

During aseptic operations, do not drag or drag with significant or rapid movements to avoid stirring up dust particles in the air.

所用的器材如培养基稀释剂配制后经121℃湿热灭菌30分钟，吸管培养皿等均应洗涤，干燥，包扎后经160℃干热灭菌2小时才能使用。

The equipment used, such as the diluent of the culture medium, should be sterilized with damp heat at 121 ℃ for 30 minutes, and the pipette culture dish should be washed, dried, and sterilized with dry heat at 160 ℃ for 2 hours before use.

无菌实验室一般设置在微生物实验室内，室内室外装备的换气设备须有空气过滤装置。在获得了无菌环境和无菌材料后，只有保持无菌状态，才能对某种特定的已知微生物进行研究。所以控菌能力和控菌稳定性是无菌室的核心验收指标。业内通行的验收标准为100级洁净区平板杂菌数平均不得超过1个菌落，10000级洁净室平均不得超过3个菌落，在通行标准之外，还应该定期保养与维护。只有建设时合理的选材指标，合适净化等级和长期管理与维护，才能使无菌实验室发挥其应有的作用。

Sterile laboratories are generally set up in microbiological laboratories, and indoor and outdoor ventilation equipment must have air filtration devices. After obtaining a sterile environment and sterile materials, only by maintaining a sterile state can a specific known microorganism be studied. So the ability and stability of bacterial control are the core acceptance indicators for sterile rooms. The acceptance standard commonly used in the industry is that the average number of miscellaneous bacteria in the 100 level clean area should not exceed 1 colony, and the average number of bacteria in the 10000 level clean room should not exceed 3 colonies. In addition to the standard, regular maintenance and upkeep should also be carried out. Only with reasonable material selection indicators, appropriate purification levels, and long-term management and maintenance during construction can sterile laboratories play their due role.

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