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  • 合理的PCR实验室设计应该注意什么?
    来源:http://www.jnyhrs.com 浏览: 发布日期:2020-10-19
    (1)各个实验区域设置合理;
    (1) Each experimental area is set up reasonably;
    (2)各个实验区域要有明显的标记(如醒目的门牌或不同的地面颜色等),以避免各个不同实验区域设备物品、试剂等发生混淆。
    (2) Each experimental area should be clearly marked (such as eye-catching door plate or different ground color) to avoid confusion of equipment, materials and reagents in different experimental areas.
    PCR实验室合理的系统设置
    Reasonable system setup of PCR Laboratory
    (1)合理的空调通风系统设置,尽量采用全送全排的空调系统;
    (1) Reasonable setting of air conditioning and ventilation system should adopt full supply and full exhaust air conditioning system as far as possible;
    (2)严格的气流压力控制,保证不同的实验区内不同的压力要求。
    (2) Strict airflow pressure control ensures different pressure requirements in different experimental areas.
    PCR实验室规范的操作
    Standard operation of PCR Laboratory
    (1)临床基因扩增检验实验室的技术人员必须进行上岗培训,经培训合格后才能从事临床基因扩增检验的工作;
    (1) The technical personnel of clinical gene amplification test laboratory must be on the job training, and can be engaged in the work of clinical gene amplification test after passing the training;
    (2)在实验操作过程中,操作者必须戴手套,并经常更换。此外,操作中使用一次性帽子也是一个有效地防止污染的措施;
    实验室装修
    (2) During the experimental operation, the operator must wear gloves and change them frequently. In addition, the use of disposable caps is also an effective measure to prevent pollution;
    (3)清洁工作及时、正确。实验工作结束后,必须立即对本区进行清洁。除常规的消毒液体对表面进行擦拭消毒或紫外线灯的照射消毒外,对一些实验设备还应进行高压消毒处理。 4.4 严格的管理
    (3) The cleaning work is timely and correct. After the experiment, the area must be cleaned immediately. In addition to conventional disinfectant liquid to wipe and disinfect the surface or ultraviolet lamp irradiation disinfection, some experimental equipment should also be treated with high pressure disinfection. 4.4 strict management
    (1)严格控制进出实验室的人员。与实验无关的人员不得随意进出实验室,有条件的情况下要设置独立的通道和进出整个实验区的门;
    (1) Strictly control the personnel entering and leaving the laboratory. Personnel not related to the experiment are not allowed to enter and leave the laboratory at will. If possible, independent channels and doors should be set up to enter and exit the entire experimental area;
    (2)在各个实验区域使用带有明显区别标志的工作服(如不同颜色),当工作人员离开时不得将本区的工作服带至其它区域;
    (2) The working clothes with obvious distinguishing marks (such as different colors) shall be used in each experimental area, and the working clothes in this area shall not be taken to other areas when the staff leave;
    (3)尽量减少在实验区内不必要的走动以减少交叉污染的可能性。
    (3) Avoid unnecessary walking in the experimental area to reduce the possibility of cross contamination.
    (4)扩增产物分析区是更主要的扩增产物污染来源,废液不能在实验室中倾倒,必须经消毒液浸泡消毒后在远离实验室的地方弃掉,用过的吸头等一次性材料也应经消毒液浸泡消毒后统一处理,如焚烧等;
    (4) The analysis area of amplification products is the main pollution source of amplification products. The waste liquid can not be dumped in the laboratory. It must be discarded in the place far away from the laboratory after being soaked and disinfected by disinfectant. The disposable materials such as used suction head should also be treated uniformly after soaking and disinfecting with disinfectant, such as incineration, etc;
    (5)扩增产物分析区可能会用到某些可致基因突变和有毒物质,应特别注意实验人员的安全防护。
    (5) Some mutagenic and toxic substances may be used in the amplified product analysis region, so special attention should be paid to the safety of laboratory personnel.
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