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  • 无尘车间的监测应该注意什么?
    来源:http://www.jnyhrs.com 浏览: 发布日期:2020-10-26
    无尘车间需要控管的项目包括温度、湿度、静压差、风速、微粒、浮游菌、沉降菌,当然在设计之初还有噪音、光照度、安全、节能等方面的考虑。对于使用者来说使用过程中学会日常监测非常重要,这是保证教学科研工作的前提之一,也有利于在出现异常数据时排查原因。
    The items that need to be controlled in the dust-free workshop include temperature, humidity, static pressure difference, wind speed, particles, planktonic bacteria, settling bacteria. Of course, at the beginning of the design, there are also considerations of noise, illumination, safety, energy conservation, etc. For users, it is very important to learn daily monitoring in the process of use, which is one of the preconditions to ensure teaching and scientific research work, and it is also conducive to find out the causes of abnormal data.
    温度、湿度、静压差、风速是预测试指标,只有这些指标都在规定范围内,后续3个主测试指标的监测才有意义。温湿度要求使用者可以自己把握,如无特殊要求时,温度在18~26℃,相对湿度在45%~65%为宜。无尘车间与非无尘车间之间的静压差应>10Pa;相邻不同洁净级别的无尘车间之间的压差应>0.5Pa;洁净区与室外压差应>12Pa。单向流净室实测室内平均风速应大于设计风速,但不应超过15%。以上测试可以通过温湿度仪、微压计、风速仪来完成。
    Temperature, humidity, static pressure difference and wind speed are the pre test indicators. Only when these indicators are within the specified range, the monitoring of the following three main test indicators is meaningful. If there is no special requirement, the temperature should be 18-26 ℃, and the relative humidity should be 45% - 65%. The static pressure difference between dust-free workshop and non dust-free workshop should be > 10Pa; the pressure difference between adjacent clean workshops with different clean levels should be > 0.5pa; the pressure difference between clean area and outdoor should be > 12Pa. The measured indoor average wind speed of unidirectional flow cleanroom should be greater than the design wind speed, but should not exceed 15%. The above tests can be completed by temperature and humidity meter, micro pressure meter and anemometer.
    无菌室装修
    悬浮粒子测试可以通过微粒计数仪来完成。监测时首先用面积开根号的方法确定少采样点数目,学校生物实验室都比较小,一般每间3~4个点就够了,采样时需注意采样点在室内均匀分布并尽量避开回风口,采样管口应正对气流方向,高度约离地80cm,测试人员应在采样口的下风侧,并尽量减少活动。为保证仪器下次能正常使用,采样完毕一定启动仪器的自净程序。
    Suspended particle test can be done by particle counter. During monitoring, the number of sampling points should be determined by the method of area root number. Generally, 3-4 points in each room are enough. When sampling, it is necessary to pay attention to the uniform distribution of sampling points in the room and avoid the air return outlet as far as possible. The sampling pipe orifice should be facing the airflow direction, with a height of about 80cm from the ground. The testing personnel should be at the downwind side of the sampling port and try to reduce activities. In order to ensure the normal use of the instrument next time, the self-cleaning program of the instrument must be started after sampling.
    浮游菌、沉降菌的采样方法及注意事项同悬浮粒子,分别通过浮游菌采样仪及带有微生物培养基的平皿来完成。培养皿在用于检测时,为避免培养皿运输或搬运过程造成的影响,宜同时进行阴性对照试验,每次或每个区域取1个对照皿,与采样皿同法操作但不需暴露采样,然后与采样后的培养皿一起放入培养箱内培养,结果应无菌落生长。
    The sampling methods and precautions for planktonic bacteria and settling bacteria are the same as those for suspended particles, which are respectively completed by planktonic bacteria sampler and plate with microbial culture medium. When the culture dish is used for testing, in order to avoid the influence caused by the transportation or transportation process of the culture dish, negative control test should be carried out at the same time. One control dish should be taken from each time or each area, and operated with the sampling dish in the same way, but without exposure sampling. Then, it should be put into the incubator together with the sampled culture dish, and the result should be aseptic colony growth.
    至于以上指标的监测频率除沉降菌外使用者可以自己把握,无尘车间启用之初应每周监测,如果数据稳定,适当延长周期,但建议至少每3个月监测1次。每次实验时超净台及无尘车间内都应当放置沉降菌平板,相当于一个实验环境的阴性对照,而对于微生物实验,尤其是细菌总数,只有在监测数据符合对应等级的无尘车间要求时数据才有效。
    As for the monitoring frequency of the above indicators, users can control it by themselves except for sedimentation bacteria. The dust-free workshop should be monitored every week at the beginning of operation. If the data is stable, the cycle should be extended appropriately, but it is recommended to monitor at least once every three months. In each experiment, the sedimentation bacteria plate should be placed in the super clean table and dust-free workshop, which is equivalent to a negative control of the experimental environment. For the microbial experiment, especially the total number of bacteria, the data is only valid when the monitoring data meet the requirements of the corresponding level of dust-free workshop.
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