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  • 无菌室的基本要求有哪些?
    来源:http://www.jnyhrs.com 浏览: 发布日期:2021-06-08
    无菌室一个应用行业非常广泛的基础性配套产业,在电子信息、半导体、医药卫生、生物工程、航天航空等众多行业均有应用,并根据行业的精 密与无尘要求,等级差别也较大。无菌室应根据既经济又科学的原则来设置。其基本要求有以几点:
    Aseptic room is a basic supporting industry widely used in electronic information, semiconductor, medical and health, bioengineering, aerospace and many other industries. According to the industry's precision and dust-free requirements, the grade difference is also large. Aseptic room should be set up according to the principle of economy and science. The basic requirements are as follows:
    一、无菌室要求
    1、 Aseptic room requirements
    1、无菌室应有内外两间,内间是无菌室,外间是缓冲室。房间容积不宜过大,以便于空气灭菌。小的内间面积2x2.5= 5m2 ,外间面积1x2= 2m2 ,高以2.5m以下为宜,都应有天花板。
    1. There should be two inside and outside aseptic rooms, the inside is aseptic room and the outside is buffer room. The room volume should not be too large for air sterilization. The small inner space area is 2x2.5 = 5m2, the outer space area is 1x2 = 2M2, the height should be less than 2.5m, and there should be ceiling.
    2、内间应当设拉门,以减少空气的波动,门应设在离工作台为远的位置上。外间好也用拉门,要设在距内间远的位置上。
    2. Sliding door should be set in the inner room to reduce the fluctuation of air, and the door should be set far away from the worktable. It's better to use sliding door in the outer room, which should be set far away from the inner room.
    3、在分隔内间与外间的墙壁或隔扇上开一个小窗,作接种过程中必要的内外传递物品的通道,以减少人员进出内间的次数,降低污染程度。小窗宽60cm、高40cm、 厚30cm,内外都挂对拉的窗扇。
    3. A small window is opened on the wall or partition fan separating the inner room from the outer room, which is used as the necessary passage for internal and external delivery of articles in the vaccination process, so as to reduce the number of people entering and leaving the inner room and reduce the degree of pollution. The small window is 60cm in width, 40cm in height and 30cm in thickness, and the inside and outside are hung with opposite sashes.
    4、无菌室容积小而严密,使用一段时间后,室内温度很高,故应设置通气窗。通气窗应设在内室进门处的顶棚上(即离工作台远的位置),好为双层结构,外层为百叶窗,内层可用抽板式窗扇。通气窗可在内室使用后、灭菌前开启,以流通空气。有条件可安装恒温恒湿机。
    4. The volume of aseptic room is small and tight. After using for a period of time, the indoor temperature is very high, so ventilation window should be set. The ventilation window should be set on the ceiling at the entrance of the inner room (i.e. the position far away from the worktable). It should be of double-layer structure, the outer layer should be louver, and the inner layer can be plate type window sash. The ventilation window can be opened after use and before sterilization to circulate air. If possible, a constant temperature and humidity machine can be installed.
    二、无菌室内设备和用具
    2、 Sterile indoor equipment and appliances
    1、无菌室内的工作台,不论是什么材质用途,都要求表面光滑和台面水平。
    1. The worktable in aseptic room, no matter what material is used, requires smooth surface and level worktable.
    2、在内室和外室各安装一个紫外灯 (多为30W )。内室的紫外线灯应安装在经常工作的座位正上方离地面2m ,外室的紫外线灯可安装在外室中 央。
    2. A UV lamp (mostly 30W) is installed in the inner room and the outer room respectively. The ultraviolet lamp in the inner room should be installed directly above the seat that works frequently, 2m above the ground, and the ultraviolet lamp in the outer room can be installed in the center of the outer room.
    3、外室应有专用的工作服、鞋、帽、口罩、盛有来苏儿水的瓷盐和毛巾、持喷雾器和5%石炭酸溶液等。
    3, the outer room should have special work clothes, shoes, caps, masks, porcelain salt and towel, spray and 5% carbolic acid solution.
    4、内室应有酒精灯、常用接种工具、不锈钢制的刀、剪、镊子、70%的酒精棉球、工业酒精、载玻璃片、特种蜡笔、记录本、铅笔、标签纸、胶水、废物筐等。
    4. There should be alcohol lamp, common inoculation tools, stainless steel knife, scissors, tweezers, 70% alcohol cotton ball, industrial alcohol, glass slide, special crayon, record book, pencil, label paper, glue, waste basket, etc.
                                           无菌室装修
    三、无菌室的灭菌消毒
    3、 Sterilization of aseptic room
    1、蒸:这是无菌室彻 底灭菌的措施。无菌室使用了较长时间,污染此较严重时,应进行薰蒸灭菌。可用甲醛、乳酸或硫磺薰蒸。
    1. Steaming: This is a measure of complete sterilization in the sterile room. When the aseptic room has been used for a long time and the pollution is serious, fumigation and sterilization should be carried out. It can be fumigated with formaldehyde, lactic acid or sulfur.
    2、喷雾:在每次使用无菌室前进行。喷雾可促使空气中微粒及微生物沉降,防止桌面、地面上的微尘飞场,并有杀菌作用。可用5%石炭酸喷雾。
    2, spray: before each use of the asepsis room. Spray can cause particles and microorganisms to settle in the air, preventing dust flying on the desktop and ground, and having bactericidal effect. It can be sprayed with 5% carbolic acid.
    3、紫外线照射:在每次使用无菌室前进行。紫外线有较好的杀菌效果。通常应开启紫外线灯照射30 ~ 60min。
    3. Ultraviolet radiation: before each use of sterile room. Ultraviolet has better germicidal efficacy. Generally, the ultraviolet lamp should be turned on for 30 ~ 60min.
    四、无菌室工作规程
    4、 Working procedure of aseptic room
    1、无菌室灭菌。每次使用前开启紫外线灯照射30min以上,或在使用前30min ,对内外室用5%石炭酸喷雾。
    1. Sterile room sterilization. Before each use, turn on the ultraviolet lamp for 30min or 30min before use, and use 5% carbolic acid spray in the interior and exterior chambers.
    2、用肥皂洗手后,把所需器材搬入外室。在外室换上已灭菌的工作服、工作帽和工作鞋,戴好口罩,然后用2%煤酚皂液将手浸洗2分钟。
    2. After washing hands with soap, move the necessary equipment into the outside room. In the outer room, put on sterilized work clothes, work caps and work shoes, wear masks, and then soak hands with 2% coal phenol soap for 2 minutes.
    3、将各种需用物品搬进内室清点就位,用5%石炭酸在工作台面上方和操作员站位空间喷雾,返回外室, 5~ 10min后再进内室工作。
    3. Move all kinds of articles into the inner room and place them in place. Spray the 5% carbolic acid on the working table and operator's space, return to the outer room, and then enter the inner room after 5~ 10min.
    4、接种操作前,用70%酒精棉球擦手。进行无菌操作时,动作要轻缓,尽量减少空气波动和地面扬尘。
    4. Before inoculation, wipe hands with 70% alcohol cotton ball. During aseptic operation, the movement should be gentle to minimize air fluctuation and dust on the ground.
    5、工作中应注意安全。如遇棉塞着火用湿布包裹熄灭,切勿用嘴吹,以免扩大燃烧。如遇有菌培养物洒落或打碎有菌容器时,应用浸润5%石炭酸的抹布包裹后, 并用浸润5%石炭酸的抹布擦拭台面或地面,用酒精棉球擦手后再继续操作。
    5. Pay attention to safety in work. If the cotton plug catches fire, wrap it with wet cloth and extinguish it. Do not blow it with your mouth to avoid expanding combustion. In case of bacteria culture spilling or breaking the bacteria container, wrap it with a cloth soaked with 5% carbolic acid, wipe the table or ground with a cloth soaked with 5% carbolic acid, and then continue the operation after wiping hands with alcohol cotton ball.
    6、工作结束后立即将台面收拾干净,将不应在无菌室存放的物品和废弃物全部拿出无菌室后,对无菌室用5%石炭酸喷雾,或开紫外线灯照射30min。
    6, after the work is finished, clean up the table immediately, and remove all the articles and wastes that should not be stored in the asepsis room, then use the 5% carbolic acid spray or the ultraviolet lamp to irradiate 30min.
    以上就是无菌室的配置要求,更多实验室相关资讯请关注我们。感谢您的阅读,希望以上内容能对您有所帮助,如您想了解更多精彩内容请点击我们的官网:无菌室装修http://www.jnyhrs.com
    The above is the configuration requirements of aseptic room. Please pay attention to us for more laboratory information. Thank you for reading, hope the above content can help you, if you want to know more wonderful content, please click our official website: aseptic room decoration http://www.jnyhrs.com .