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  • 细胞培养实验室要注意哪些工作?
    来源:http://www.jnyhrs.com 浏览: 发布日期:2021-10-13
    理想的无菌操作室应划为三部分:
    The ideal sterile operation room should be divided into three parts:
    ●更衣室:供更换衣服、鞋子及穿戴帽子和口罩。
    ● dressing room: for changing clothes, shoes, hats and masks.
    ●缓冲间:位于更衣间与操作间之间。
    ● buffer room: located between dressing room and operation room.
    目的是为了保证操作间的无菌环境,同时可放置恒温培养箱及某些必需的小型仪器。
    The purpose is to ensure the sterile environment of the operation room, and place the constant temperature incubator and some necessary small instruments at the same time.
    ●无菌操作间:专用于无菌操作、细胞培养。
    ● aseptic operation room: dedicated to aseptic operation and cell culture.
    大小要适当,且其顶部不宜过高(不超过2.5m),以保证紫外线的有效灭菌效果;
    The size shall be appropriate and the top shall not be too high (no more than 2.5m) to ensure the effective sterilization effect of ultraviolet light;
    墙壁光滑无死角以便清洁和消毒;
    The wall is smooth without dead corners for cleaning and disinfection;
    济南沙发套定做
    工作台安置不应靠墙壁,台面要光滑压塑作表面,漆成白色或灰色以利于解剖组织及酚红显示pH的观察。
    The worktable shall not be placed against the wall, and the surface of the worktable shall be smooth, molded and painted white or gray, so as to facilitate the observation of anatomical tissue and phenol red to show pH.
    孵育区——本区对无菌的要求虽不比无菌区严格,但仍需清洁无尘,因此也应设置在干扰少而非来往穿行的区域。孵育可在孵箱或可控制温度的温室中进行,后者费用高,一般实验室多采用孵箱进行工作。
    Incubation area - although the requirements for sterility in this area are not more strict than those in the sterile area, it still needs to be clean and dust-free. Therefore, it should also be set in the area with less interference rather than passing through. Incubation can be carried out in incubators or temperature controlled greenhouses, which are expensive. Generally, incubators are used in laboratories.
    储藏区——主要存放各类冰箱、干燥箱、液氮罐、无菌培养液、培养瓶等,环境需要清洁无尘。
    Storage area - it mainly stores all kinds of refrigerators, drying boxes, liquid nitrogen tanks, sterile culture solutions, culture bottles, etc. the environment needs to be clean and dust-free.
    制备区——主要进行培养液及有关培养用的液体等的制备。
    Preparation area - it is mainly used for the preparation of culture medium and related liquid for culture.
    清洗和消毒灭菌区——清洁和消毒灭菌区应与其他区域分开,主要进行所有细胞培养器皿的清洗、准备、消毒及三蒸水制备等工作。
    Cleaning, disinfection and sterilization area - the cleaning, disinfection and sterilization area shall be separated from other areas, mainly for the cleaning, preparation, disinfection and preparation of three distilled water of all cell culture vessels.