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  • 无菌室的实验操作要注意什么?
    来源:http://www.jnyhrs.com 浏览: 发布日期:2021-10-25
    实验进行前,无菌室及无菌操作台以紫外灯照射30-60分钟灭菌,以70%的酒精擦拭无菌操作抬面,并开启无菌操作台风扇运转10分钟后,才开始实验操作。
    Before the experiment, the sterile room and the sterile console are irradiated with ultraviolet lamp for 30-60 minutes for sterilization, the sterile operation lifting surface is wiped with 70% alcohol, and the fan of the sterile console is turned on for 10 minutes before the experimental operation is started.
    小心取用无菌之实验物品,避免造成污染。勿碰触吸管尖头部或是容器瓶口,亦不要在打开之容器正上方操作实验。容器打开后,以手夹住瓶盖并握住瓶身,倾斜约45°角取用,尽量勿将瓶盖盖口朝上放置桌面。
    Take sterile test articles carefully to avoid contamination. Do not touch the tip of the pipette or the mouth of the container, and do not operate the experiment directly above the open container. After the container is opened, clamp the bottle cap and hold the bottle body by hand, tilt it at an angle of about 45 °, and try not to place the opening of the bottle cap upward on the table.
    工作人员应注意自身之安全,须穿戴实验衣及手套后才进行实验。对于来自人类或是病毒感染之细胞株应特别小心操作,并选择适当等级之无菌操作台(至少ClassII)。操作过程中,应避免引起气溶胶类物质的产生,小心毒性药品,例如DMSO及TPA等,并避免尖锐针头之伤害等。
    Staff should pay attention to their own safety and carry out the experiment only after wearing experimental clothes and gloves. For human or virus infected cell lines, special care should be taken and an appropriate level of sterile console (at least class II) should be selected. During operation, avoid the generation of aerosol substances, be careful of toxic drugs, such as DMSO and TPA, and avoid the injury of sharp needles.
    接种针每次使用前后,必须通过火焰灼烧灭菌,待冷却后,方可接种培养物。
    Before and after each use, the inoculation needle must be burned and sterilized by flame. After cooling, the culture can be inoculated.
    无菌室装修
    无菌操作工作区域应保持清洁及宽敞,必要物品,例如试管架、吸管吸取器或吸管盒等可以暂时放置,其它实验用品用完即应移出,以利于气流之流通。实验用品以70%的酒精擦拭后才带入无菌操作台内。实验操作应在抬面之无菌区域,勿在边缘的非无菌区域操作。
    The sterile operation work area shall be kept clean and spacious. Necessary items, such as test tube rack, pipette aspirator or pipette box, can be placed temporarily. Other experimental supplies shall be removed after use to facilitate the flow of air. The experimental articles are wiped with 70% alcohol before being brought into the sterile operation table. The experimental operation should be carried out in the sterile area in the center of the lifting surface, not in the non sterile area at the edge.
    使用无菌瓶内的溶液是,不可将无菌敷料堵塞瓶口倾倒无菌溶液,或直接伸入溶液瓶内蘸取,以免污染剩余的溶液。
    When using the solution in the sterile bottle, do not block the bottle mouth with sterile dressing, pour the sterile solution, or directly dip it into the solution bottle to avoid polluting the remaining solution.
    每次操作过程中,均应做阴性对照,以检查无菌操作的可靠性。
    During each operation, a negative control shall be made to check the reliability of aseptic operation.
    如有菌液洒在桌上或地上,应立即用5%石碳酸溶液或3%的来苏尔倾覆在被污染处至少30分钟,再做处理。工作衣帽等受到菌液污染时,应立即脱去,高压蒸汽灭菌后洗涤。
    If bacterial liquid is spilled on the table or the ground, it shall be immediately overturned at the contaminated place with 5% carbolic acid solution or 3% Lysol for at least 30 minutes before treatment. When the working clothes and hats are contaminated by bacterial solution, they shall be removed immediately and washed after high-pressure steam sterilization.
    无菌包内物品不慎污染或无菌包浸湿,外界微生物可渗入包内,造成污染,需重新消毒。
    If the articles in the sterile bag are accidentally polluted or the sterile bag is soaked, external microorganisms can penetrate into the bag, causing pollution, which needs to be disinfected again.
    凡带有活菌的物品,必须经消毒后,才能在水龙头下冲洗,严禁污染下水道。
    All articles with live bacteria must be disinfected before they can be washed under the faucet. It is strictly prohibited to pollute the sewer.
    吸取菌液时,必须用吸耳球吸取,切勿直接用口接触吸管。
    When sucking bacterial liquid, you must use an ear ball to suck it. Do not directly touch the straw with your mouth.
    带有菌液的吸管,试管,培养皿等器皿应浸泡在盛有5%来苏尔溶液的消毒桶内消毒,24小时后取出冲洗。
    Straws, test tubes, Petri dishes and other utensils with bacterial solution shall be soaked in a disinfection barrel containing 5% Lysol solution for disinfection, and taken out for flushing after 24 hours.
    戴手套时应注意未戴手套的手不可触及手套外面,而戴手套的手则不可触及未戴手套的手或另一手套的里面。戴手套后如发现破裂,应立即更换。脱手套时,须将手套口翻转胶下,不可用力强拉手套边缘或手指部分,以免损坏。
    When wearing gloves, it should be noted that the hand without gloves cannot touch the outside of the glove, while the hand with gloves cannot touch the hand without gloves or the inside of another glove. If the gloves are found broken after wearing, they should be replaced immediately. When taking off the glove cover, turn the glove mouth under the glue, and do not force the edge or finger of the glove to avoid damage.
    实验完毕后,将实验物品带出工作台,以70%的酒精擦拭无菌操作抬面。操作间隔应让无菌操作台运转10分钟以上后。
    After the experiment, take the experimental articles out of the workbench and wipe the sterile operation surface with 70% alcohol. The operation interval shall be after the sterile operation table has operated for more than 10 minutes.
    总的来说,实验室装修不是普通的工装装修,有自己特定的标准和规范,无菌室设计装修有很多细节需要注意,在装修中必须综合考虑实验室整体规划合理布局。当前无菌室多存在于微生物工厂,一般实验室则使用超净台。超净台其主要功能是利用空气层流装置排除工作台面上部包括微生物在内的各种微小尘埃。通过电动装置使空气通过过滤器具后进入工作台面,使台面始终保持在流动无菌空气控制之下。而且在接近外部的一方有一道高速流动的气帘防止外部带菌空气进入。随着实验室装修水平的提高,实验室工作人员对设计的要求越来越高,
    In general, the laboratory decoration is not an ordinary tooling decoration, but has its own specific standards and specifications. There are many details to pay attention to in the design and decoration of the sterile room. In the decoration, we must comprehensively consider the overall planning and reasonable layout of the laboratory. At present, most aseptic rooms exist in microbial factories, and ultra clean tables are used in general laboratories. The main function of the super clean table is to use the air laminar flow device to remove all kinds of micro dust including microorganisms on the upper part of the worktable. Through the electric device, the air enters the worktable after passing through the high-efficiency filter, so that the worktable is always under the control of flowing sterile air. Moreover, there is a high-speed air curtain on the side close to the outside to prevent the entry of external bacteria carrying air. With the improvement of laboratory decoration level, laboratory staff have higher and higher requirements for design,
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    The above is the decoration of sterile room http://www.jnyhrs.com Thank you for checking the information of our company in your busy schedule. If you want to know more, you are welcome to call for consultation!